Myocardial ischaemia inhibits mitochondrial metabolism of 4-hydroxy-trans-2-nonenal

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Myocardial ischaemia inhibits mitochondrial metabolism of 4-hydroxy-trans-2-nonenal.

Myocardial ischaemia is associated with the generation of lipid peroxidation products such as HNE (4-hydroxy-trans-2-nonenal); however, the processes that predispose the ischaemic heart to toxicity by HNE and related species are not well understood. In the present study, we examined HNE metabolism in isolated aerobic and ischaemic rat hearts. In aerobic hearts, the reagent [(3)H]HNE was glutath...

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Trans-4-oxo-2-nonenal potently alters mitochondrial function.

Alzheimer disease elevates lipid peroxidation in the brain and data indicate that the resulting lipid-aldehydes are pathological effectors of lipid peroxidation. The disposition of 4-substituted nonenals derived from arachidonate (20:4, n-6) and linoleate (18:2, n-6) oxidation is modulated by their protein adduction targets, their metabolism, and the nature of the 4-substitutent. Trans-4-oxo-2-...

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Cyclooxygenase-2 generates the endogenous mutagen trans-4-hydroxy-2-nonenal in Enterococcus faecalis-infected macrophages.

Infection of macrophages by the human intestinal commensal Enterococcus faecalis generates DNA damage and chromosomal instability in mammalian cells through bystander effects. These effects are characterized by clastogenesis and damage to mitotic spindles in target cells and are mediated, in part, by trans-4-hydroxy-2-nonenal (4-HNE). In this study, we investigated the role of COX and lipoxygen...

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Cytotoxicity and metabolism of 4 - hydroxy - 2 - nonenal and 2 - nonenal in H 202 - resistant cell lines

Toxic aldehydes, such as 4-hydroxy-2-nonenal (4HNE) and 2-nonenal (2NE), formed during lipid peroxidation have been isolated and implicated in the cytotoxic effects of oxidative stress. We have investigated the cytotoxicity and metabolism of 4HNE and 2NE in control (HA-1) cells and in two H202-resistant Chinese hamster fibroblast cell lines. The H202resistant cells were found to be significantl...

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Mass spectrometric characterization of covalent modification of human serum albumin by 4-hydroxy-trans-2-nonenal.

Several pieces of evidence indicate that albumin modified by HNE is a promising biomarker of systemic oxidative stress and that HNE-modified albumin may contribute to the immune reactions triggered by lipid peroxidation-derived antigens. In this study, we found by HPLC analysis that HNE is rapidly quenched by human serum albumin (HSA) because of the covalent adduction to the different accessibl...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2008

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj20081615